Relationship between factor V Leiden mutation and poor pregnancy outcomes

Relationship between factor V Leiden mutation and poor pregnancy outcomes PDF

Khaled Shelbayeh

Supervisor(s)

Dr. Ayman Hussein -

Discussion Commity

1-Dr. Ayman Hussein (Supervisor) 2-Dr. Hesham Darwish (Internal examiner) 3-Dr.Ala`a Salah (External examiner)

83 صفحة

Abstract :

Abstract Pregnancy is a hypercoagulable state, with increased tendency for thrombus formation. That is increased if pregnancy is combined with thrombophilia. Thrombophilia could be acquired or inherited. Among the inherited types is Factor V Leiden mutation, an autosomal dominant disorder. The mutation is believed to be a major inherited risk factor for venous thrombosis. Recently, it was suggested that this mutation, through stimulation of venous and placental thrombosis events, were strongly associated with different pregnancy adverse outcomes, including PET, recurrent miscarriages, IUGR, IUFD, abruption placenta, and others. Although other studies disputed such a link. The aim of our study was to investigate the relationship between Factor V Leiden mutation and some adverse pregnancy outcomes, namely recurrent miscarriages and PIH. In this case-control study where 137 participants with adverse pregnancy outcomes (66 First trimester RM, 25 second trimester RM, 26 with IUFD & 20 with PIH) were compared to 155 women with uncomplicated pregnancies. Blood samples were collected from participants for DNA extraction; and Factor V Leiden mutation was identified using PCR The mutation was confirmed in 35 cases out of 137 (25.5%), and in 13 out of 155 controls (8.4%). .The relationship between the mutation and recurrent miscarriages was established using SPSS analysis version 15 [Odds ratio was 3.75 p-value=0.000] Comparing those with first trimester abortion cases and the control group odds ratio was 2.45 p-value=0.029, while the ratio was 10.5 and p value was 0.00 when comparing those with second trimester abortions cases to the control, a comparison between women with IUFD and the control group in relative to the Factor V Leiden mutation, the odds ratio was 4.5 and p-value was 0.000. Finally comparing women with PIH to controls, in relative to the prevalence of Leiden mutation no significant difference existed between the two groups These results suggest a strong correlation between recurrent miscarriages and Factor V mutation in our population.

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